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Schematic representation of EGFP and flagellin-EGFP fusion protein. EGFP and flagellin-EGFP were synthesized as described in Materials and Methods. (A) Diagram of EGFP and flagellin-EGFP proteins. a.a., amino acids. (B) An aliquot of each protein was loaded onto a 10% polyacrylamide gel and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Molecular weight markers (M.W.M) are indicated in thousands.
