FIG. 5.

APCs pulsed with flagellin-EGFP process and present EGFP. APCs cells were pulsed for 18 h with Ad-EGFP, flagellin-EGFP, or EGFP or were unpulsed. The cytotoxic activity of an anti-EGFP-specific CD8⁺-T-cell line was analyzed against the pulsed APCs in a 6-h ⁵¹Cr release assay at the indicated ratios of effector to target cells (E:T). Data are the means of two independent experiments (performed with triplicates) (error bars, standard deviations). A significant (P ≤ 0.001 [Student's t test]) difference was found between the control group and the flagellin-EGFP group.