. 2013 Dec 5;13:203. doi: 10.1186/1471-2229-13-203

Table 5.

Sequences of the species-specific primers that amplify a part of the ITS1 region

*Pythium* isolate	Forward ITS primer (5′-3′)^a	Reverse ITS primer (5′-3′)^a	Size amplicon	GenBank Accession no.^b
P. arrhenomanes PT 60	ATTCTGTACGCGTGGTCTTCCG (3 μM)	ACCTCACATCTGCCATCTCTCTCC (1 μM)	311 bp	HQ877857
P. graminicola PB912 132	ATGGCTGAACGAAGGTGGGCTG (1 μM)	TCCCGAAAGTGCAATGTGCGTTC (3 μM)	240 bp	HQ877865
P. inflatum PT 52	AGGTGGGCGCATGTATGTGTGTC (500 nM)	ACGTATCGCAGTTCGCAGCG (3 μM)	165 bp	HQ877856

^aThe concentrations for each primer stock are presented between brackets.

^bThe ITS-regions of P. arrhenomanes PB912 75 (HQ877857) and P. graminicola PB912 116 (HQ877865) are respectively identical to those of P. arrhenomanes PT 60 and P. graminicola PB912 132 [3].