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. 2013 Dec 5;13:47. doi: 10.1186/1471-2253-13-47

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Copyright © 2013 Sakuramoto et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression level of potential inflammatory cytokines as revealed by ELISA and Real Time PCR. Serum and pulmonary levels of interleukin (IL)-6 and tumor necrosis factor (TNF) –α at the end of the study by ELISA (A-D). The mRNA expression of IL-6 and TNF-α at the end of the study by Real Time PCR (E-F). OS, open endotracheal suctioning; CS, closed endotracheal suctioning; Control, control group with ARDS, but without endotracheal suctioning; HC, healthy control group with 6 hour ventilation, but without ARDS and endotracheal suctioning. *p < 0.05, compared with healthy control (HC) group.