TABLE 1.
ELISA Ab levels in sera from mice or rabbits immunized with protein BVH-3 or BVH-11 or their respective truncated regions or with the chimeric proteina
| Animal | Immunogen | ELISA Ab level with antigen
|
|||||
|---|---|---|---|---|---|---|---|
| BVH-3M | BVH-3C | BVH-11M | BVH-11B | Chimeric CH3-C-11B | RX-1 cells | ||
| Mouse | BVH-3M | 2,128 | 834 | 513 | 7 | 816 | 45 |
| BVH-3-SP63 | 1,853 | 753 | 386 | 5 | 736 | 17 | |
| BVH-3A | 2,867 | 44 | 567 | 11 | 22 | 18 | |
| BVH-3B | 1,527 | 1,197 | 17 | 3 | 1,662 | 27 | |
| BVH-11M | 828 | 37 | 2,491 | 1,705 | 2,993 | 97 | |
| BVH-11B | 10 | 33 | 3,016 | 3,816 | 5,431 | 113 | |
| Thioredoxin | 5 | <2 | 9 | <2 | 2 | 5 | |
| Rabbitb | BVH-3M | >128,000 | >128,000 | 32,000 | <100 | >128,000 | 32,000 |
| L-BVH-3A | >128,000 | 8,000 | >128,000 | 8,000 | 8,000 | 32,000 | |
| BVH-3C | >128,000 | >128,000 | 100 | 4,000 | >128,000 | 64,000 | |
| L-BVH-11 | >128,000 | 8,000 | >128,000 | >128,000 | >128,000 | >128,000 | |
| BVH-11B | 400 | 400 | >128,000 | >128,000 | >128,000 | 32,000 | |
| CH-3C-11B | >128,000 | >128,000 | >128,000 | >128,000 | >128,000 | >128,000 | |
a
Post-dose-3 sera were tested. Mouse Ab levels are expressed in micrograms per milliliter, while rabbit Ab levels are expressed as titers as defined in Materials and Methods.
b
All rabbit preimmune sera had anti-protein and anti-RX-1 Ab titer values of <100 and ≤800, respectively.