FIG. 5.

IL-12 treatment decreased total cell numbers in lungs of TB-infected mice. Total numbers of CD8⁺, CD4⁺, and NK⁺ (CD49b/Pan-NK) cells were determined by flow cytometry of total cells obtained from lung homogenates. Squares, IL-12 treatment; circles, PBS treatment. CD8⁺ (A), CD4⁺ (B), and NK (C) cell numbers in whole lungs were reduced in IL-12-treated mice compared to saline controls. Each data point represents three to four mice and the bars represent the standard errors. CD4⁺ and NK⁺ graphs are representative of CD4^−/− mice from three experiments and WT mice from four experiments; CD8⁺ graphs are representative of CD4^−/− mice from two experiments and WT mice from three experiments. In the above experiment with CD4^−/− mice, total cell numbers from whole lungs were not available for early times postinfection. *, P ≤ 0.05; **, P ≤ 0.01.