TABLE 2.
Numbers of immune cells within the lungs at 44 days postinfectiona
| TB-infected mice and treatment | Total no. of cells (104) | No. of cells (104)
|
|||||
|---|---|---|---|---|---|---|---|
| CD8+ | CD4+ | NK+ | Neutrophils | Macrophages | IFN-γ+ | ||
| WT plus IL-12 | 313 ± 79 | 20.4 ± 7.7 | 18.0 ± 5.7 | 9.0 ± 2.8 | 6.1 ± 1.9 | 22.8 ± 7.8 | 1.1 ± 0.3 |
| WT plus saline | 973 ± 343 | 52.1 ± 13.8 | 74.8 ± 26.0* | 29.1 ± 7.8* | 16.4 ± 6.2 | 71.7 ± 26.5 | 4.1 ± 1.9 |
| CD4−/− plus IL-12 | 403 ± 66 | 24.3 ± 3.0 | NA | 9.0 ± 3.8 | 10.9 ± 3.3 | 26.8 ± 4.8 | 0.8 ± 0.4 |
| CD4−/− plus saline | 717 ± 54** | 52.6 ± 5.3** | NA | 21.8 ± 1.8* | 22.2 ± 2.1* | 55.7 ± 5.5** | 4.6 ± 0.6** |
a
Numbers of total cells, CD8+ lymphocytes, CD4+ lymphocytes, natural killer cells, neutrophils, macrophages, and IFN-γ producers at 44 days postinfection were calculated. Cell numbers are means ± standard errors. Each value represents the mean of three or four mice. The data are representative of three experiments with CD4−/− mice and four experiments with WT mice. For this experiment, B cells were not studied. *, P < 0.05; **, P < 0.01 when comparing treatments within WT or CD4−/− groups. NA, not applicable.