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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Neurobiol Aging. 2012 Jun 6;34(2):10.1016/j.neurobiolaging.2012.05.003. doi: 10.1016/j.neurobiolaging.2012.05.003

Figure 4.

Figure 4

Measuring extracellular tau in cultured cells. A–B. Human neuronal SH-SY5Y cells express endogenous human tau. A. Extracellular tau was measured with ELISAs specific to total, pT181, and pS396 tau. B. Intracellular tau was measured with ELISAs specific to total, pT181, and pS396 tau. C–D. HEK293-T cells, which do not express tau, were transiently transfected with 1 ug human tau (longest isoform: 2N4R) for 24 hrs. C. Extracellular tau was measured with ELISAs specific to total, pT181, and pS396 tau. D. Intracellular tau was measured with ELISAs specific to total, pT181, pS396 tau. Values are expressed relative to total intracellular protein, as measured by a BCA assay. Image shown is representative of six replicate experiments.