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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Neurobiol Aging. 2012 Jun 6;34(2):10.1016/j.neurobiolaging.2012.05.003. doi: 10.1016/j.neurobiolaging.2012.05.003

Figure 5.

Figure 5

Extracellular tau is predominantly full-length. A. Media from HEK293-T cells transiently expressing human tau (2N4R) was isolated and analyzed by SDS-PAGE. Immunoblots were probed with antibodies specific to the N-terminus (Tau12), central domain (Tau1), and C-terminus (Tau7) of tau. B. Media from HEK293-T cells expressing human tau (2N4R) was immunoprecipitated with antibodies specific to the N-terminus (Tau12) and C-terminus (Tau7) of tau. Immunoblots were probed with an antibody specific to the N-terminus (Tau12) of tau. Images shown are representative of four replicate experiments.