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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Neurobiol Aging. 2012 Jun 6;34(2):10.1016/j.neurobiolaging.2012.05.003. doi: 10.1016/j.neurobiolaging.2012.05.003

Figure 7.

Figure 7

Overexpression of calcineurin subunits reduces extracellular tau levels. HEK293-T cells were transiently transfected with vectors containing human tau (2N4R) and GFP, PPP3R1, and/or PPP3CA cDNA. Media and cell lysates were collected and analyzed for total tau and phospho-tau levels. A. Cell lysates were analyzed by SDS-PAGE and immunoblots were probed with antibodies directed to calcineurin subunits. B. Calcineurin activity in cell lysates. C. Intracellular total tau was measured in cell lysates with an ELISA specific to human total tau. D. Intracellular pT181 was measured in cell lysates with an ELISA specific to human pT181. E. Extracellular total tau was measured in cell media with an ELISA specific to human total tau. F. Extracellular pT181 was measured in cell media with an ELISA specific to human ptau181. *p<0.01. Image shown is representative of four replicate experiments.