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. 2013 Nov 23;13:326. doi: 10.1186/1472-6882-13-326

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Copyright © 2013 Mamat et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The HPLC analysis of MEMM. A) The HPLC profile of MEMM at the wavelength of 366 nm. B) The UV spectra analysis of MEMM at 366 nm. The chromatogram demonstrated the presence of several peaks, with the most major peak detected at the retention time (RT) of 28.584 min. This peak was observed at the λmax within the region of 284.9-349.4 nm, suggesting, in part, the presence of flavonoid-based compounds. C) The HPLC chromatogram of MEMM shows the presence of quercitrin (1) and rutin (2) at 366 nm.