Table 3.
Recovery of artemether and dihydroartemisinin.
| Analyte | Concentration (ng/ml) |
Recovery (%) | |
|---|---|---|---|
| With 1% formic acid | Without acidification | ||
| ARM | 1.5 | 116 | 52.2 |
| 170 | 122 | 62.7 | |
| DHA | 1.5 | 100 | 83.1 |
| 170 | 103 | 98.1 | |
Recovery with 1% formic acid was obtained by mixing low and high QC samples in triplicate with internal standard solution containing 1% formic acid and then processing with HLB micro-elution plate. Recovery without acidification was obtained by mixing low and high QC samples in triplicate with internal standard solution that was not acidified.
ARM: Artemether; DHA: Dihydroartemisinin.