Figure 4. Translational control in the long-lived ER secretory pathway mutants.

(A) Changes in protein translation in alg12Δ, bst1Δ, and wild-type cells treated with tunicamycin (TM) relative to untreated wild-type cells are shown in log₂ scale for all genes that are activated or repressed more than 1.5-fold in at least one of the strains. (B) Cluster analysis of log2 TE changes in alg12Δ, bst1Δ, and TM treated cells relative to untreated wild-type cells. Changes in log2 TE are shown for all genes that showed more than 1.5-fold decrease or increase in TE. (C) Polysome profiles of alg12Δ, bst1Δ, and TM treated cells. Long-lived deletion strains alg12Δ and bst1Δ do not show overall translational suppression.