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. 2014 Jan 2;10(1):e1003954. doi: 10.1371/journal.pgen.1003954

Figure 1. The adp1-D mutant displayed pleiotropic phenotypes.

Figure 1

(A) Twelve-day-old seedlings and (B) 28-day-old plants grown under long-day conditions. The symbol +/− represents heterozygous mutants, while −/− represents homozygous mutants. (C) Emergence rate of rosette leaves in wild-type and adp1-D plants. At least 20 plants were measured for each genotype. (D) Six-week-old plants grown under long-day conditions. (E) Schematic diagram of Arabidopsis branching pattern. CI, first order cauline branches; CII, higher order cauline branches; RI, first order rosette branches; RII, higher order rosette branches. (F) First-order rosette branch number of two-month-old wild-type and adp1-D plants. At least 40 plants of each genotype were measured. (G) and (H) Detection of cotyledon petiole from 20-day-old plants of (G) the wild type and (H) the adp1-D mutant. Although no axillary bud was produced in the cotyledon axil in wild-type plants, about 30% of the adp1-D mutants produced an inflorescence in the cotyledon axil (indicated by arrowheads). (I) Generation rate of rosette branches in wild-type and adp1-D plants. At least 20 plants were measured for each genotype. Axillary buds longer than 2 mm were considered as axillary branches. (J) to (O) Scanning electron micrographs of developing axillary buds in the axils of the first pair of rosette leaves of wild-type (J–L), and adp1-D (M–O) plants. Plants were fixed after 14–18 days growth under long-day conditions. Development of the axillary buds can be divided into three stages. In stage 1, the axillary shoot bulged outwards, forming a semicircular zone. In stage 2, the outgrowth increased in size, forming an axillary leaf primordia. In stage 3, the axillary buds start to grow outwards. Under the growth conditions in this study, only one primordium was formed in the leaf petiole axil in wild-type plants, and most of the primordia stagnate in stage 2, whereas the axillary buds in adp1-D mutants developed faster (comparing K with N) and were at more advanced stages (comparing L with O). For (A) (B) and (D), bar = 1 cm; for (G) and (H), bar = 1 mm; for (J) to (O), bar = 40 µm.