Figure 7. Correlation of auxin level with first-order rosette branch number.
(A) Morphology of 35-day-old plants of adp1-D, Pro35S:YUCCA1 and double mutants of adp1-D Pro35S:YUCCA1. Bar = 1 cm. (B) Quantitative analysis of first-order rosette branch from 2-month-old plants of adp1-D, Pro35S:YUCCA1 and double mutants of adp1-D Pro35S:YUCCA1. Thirty plants of each genotype were measured. The error bars represent the SD. (C) Quantitative analysis of emergence rate of rosette leaves in adp1-D, Pro35S:YUCCA1 and double mutants of adp1-D Pro35S:YUCCA1. Thirty plants of each genotype were measured. The error bars represent the SD. (D) to (I) Morphology of crossed lines between adp1-D and ProADP1:iaaM transgenic lines at 25 days after germination (D to F) and two months after germination (G to I). The axillary buds developed much slower in the crossed lines compared with adp1-D, indicated by arrowheads from (D) to (F). At maturity, the crossed lines with stronger iaaM expression level even showed terminated flowers, as indicated by the inserted frame in (I). (J) Expression quantity of iaaM in the wild type and the crossed lines shown in (D) to (I) analyzed by real-time-quantitative PCR (qPCR). (K) First-order rosette branch number of the wild type and the crossed lines shown in (D) to (I). (L) Emergence rate of rosette leaves in the wild type and the crossed lines shown in (D) to (I).