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. 2014 Jan 2;10(1):e1004004. doi: 10.1371/journal.pgen.1004004

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© 2014 Hégarat et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Experimental protocol: DT40 cdk1as cells are blocked in G2 phase by 1NMPP1. Mitosis is triggered either by removing 1NMPP1 from the media, or by treating the cells with 1 µM OA. (B) Immuno-fluorescence analysis of cdk1as cells before (0) and 5 minutes after (5) release from G2 by 1NMPP1 removal using DAPI and the indicated antibodies. (C) Immuno-fluorescence analysis of mitotic entry in 1NMPP1 arrested cdk1as cells at indicated timepoints after treatment with 1 µM OA. (D) Immuno-blot analysis of 1NMPP1 treated cdk1as cell extracts taken at indicated timepoints following OA treatment, or from cells treated with both 1 µM OA and 10 µM TC.