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. 2013 Dec 26;11:317. doi: 10.1186/1479-5876-11-317

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Copyright © 2013 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of E2A is decreased in metastatic CRCs. A, Relative E2A mRNA expression in primary CRCs with or without metastasis. The levels of E2A mRNA were examined using qRT-PCR by the 2^-ΔCT method with GAPDH mRNA as internal control. B, E2A protein expression in primary CRCs with or without metastasis was detected with immunoblot using GAPDH protein as loading control. Protein samples of lane 1, 3, 5, 7, 9 and 11 were from tumors without metastasis, while lane 2, 4, 6, 8, 10 and 12 were from tumors with metastasis. Samples were selected and paired randomly. Data represents means ± SD from 3 independent experiments. **, P < 0.01.