Table 1. Mutation in Sfswap results in fewer hair cells.
Cochlear Region | Row | WT Count | Tg/Tg Count | P value |
Apex | IHC | 24.2+/−0.49 | 37.2+/−3.10 | 0.003 |
Row1 OHC | 27.4+/−0.51 | 27.6+/−1.78 | NS | |
Row2 OHC | 28.4+/−0.51 | 27.6+/−1.91 | NS | |
Row3 OHC | 28.8+/−0.37 | 16.4+/−2.54 | 0.0013 | |
Middle | IHC | 24.8+/−0.37 | 22.2+/−0.58 | 0.006 |
Row1 OHC | 26.0+/−0.95 | 27.2+/−0.86 | NS | |
Row2 OHC | 26.2+/−0.66 | 26.0+/−0.55 | NS | |
Row3 OHC | 26.6+/−0.75 | 3.0+/−1.48 | 5.9E-7 | |
Base | IHC | 22.4+/−0.68 | 21.6+/−0.81 | NS |
Row1 OHC | 24.5+/−0.65 | 25+/−0.58 | NS | |
Row2 OHC | 25.2+/−0.80 | 25.8+/−0.80 | NS | |
Row3 OHC | 25.6+/−1.17 | 13.0+/−2.53 | 0.0088 | |
Whole Cochlea | IHC | 644+/−10.5 | 402+/−5.03 | 1.6E-4 |
OHC | 2066+/−29 | 1021+/−26.2 | 1.2E-4 |
The number of hair cells in each row per 200 µm was collected at the apex, mid and base in P0 cochleas. In SfswapTg/Tg mutants, the third row of outer hair cells is reduced in all areas. Loss is greatest in the middle cochlea. The number of inner hair cells is significantly increased at the apex. The total number of hair cells was counted in WT and Tg/Tg cochleas at P2 and mutants show a significant decrease in both inner and outer hair cells.