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. 2014 Jan 2;9(1):e82087. doi: 10.1371/journal.pone.0082087

Figure 4. Transcriptional activity of Tktl1-promoter deletion mutants in one-cell-stage embryos.

Figure 4

(A) The upstream region of the Tktl1 promoter (Tktl1-2467+25) was deleted to leave 227 bp (Tktl1-227+25) and 56 bp (Tktl1-56+25), and transcriptional activity was then evaluated. (B) The 25 bp downstream of the TSS was deleted from pTktl1-56+25 and transcriptional activity was measured. Plasmids (500 ng/µl) were microinjected into the male pronuclei of one-cell-stage embryos. The experiments were performed at least 11 times and the results are presented as means ± SEM. Asterisks indicate significant differences (Student's t-test; P<0.05).