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. 2014 Jan 2;9(1):e83417. doi: 10.1371/journal.pone.0083417

Table 1. Serum antibody titers and viral detection in the H5N1 HPAI virus-inoculated (group 1) and acute stage contact (group 2) ducks.

Pre-Inoculation 8 dpi 15 dpi
Bird ID ELISA HI-C HI-H VN ELI SA HI-C HI-H VN ELI SA HI-C HI-H VN VIa rRT-PCRb
Group 1
#73 36 <4 <4 <4 107 16 32 <4 104 128 128 128 + 27.8
#75 26 <4 <4 <4 103 8 32 <4 103 64 128 128 + 23.6
#77 34 <4 <4 <4 99 16 64 <4 105 128 128 128 + 26.8
#83 36 <4 <4 <4 101 32 64 <4 99 256 512 128 + 21.6
#92 30 <4 <4 <4 104 16 16 <4 104 128 256 128 + 24.5
Group 2
#71 27 <4 <4 <4 55 <4 <4 <4 63 <4 <4 <4 + 30.5
#84 17 <4 <4 <4 54 <4 <4 <4 104 128 256 16 + 21.5
#85c 37 <4 <4 <4 102 <4 <4 <4 93 4 16 <4 + 24.7
#87 33 <4 <4 <4 68 <4 <4 <4 90 64 128 32 + 26.4
#94c 26 <4 <4 <4 64 <4 <4 <4 nd nd nd nd + 30.9

Five ducks inoculated with H5N1 HPAI virus (group 1) and five naive contact ducks (group 2) were mixed at one day post inoculation (dpi). Sera were collected at 4 days pre-inoculation for both groups and at 8 and 15 dpi (equivalent to 7 and 14 days post contact (dpc) respectively for group 2). Sera were tested with blocking ELISA (values are % inhibition; cut-off = 60%), HI using chicken RBCs (HI-C) and horse RBCs (HI-H), and virus neutralization (VN) tests. nd: not done.

a Summary of virus isolation (VI): a+symbol indicates that virus was isolated at least once from either oral or cloacal swab from the duck during the duration of the trial.

b Summary of Ct values for real-time reverse transcription polymerase chain reaction (rRT-PCR) results: the value is the lowest detected for the duck from either oral or cloacal swabs during the duration of the trial.

c Ducks 94 and 85 developed disease during the experiment; therefore the last sera were obtained when they were euthanized, for welfare reasons, at 5 dpc (shown under 8 dpi) and 9 dpc (shown under 15 dpi), respectively.