Figure 3. Antigen-specific IL-2 production in naïve and vaccinated mice, as tested by ELISA.

Interleukin-2 levels in 24 hr spleen cell culture supernatants of C57BL/6 mice vaccinated against the 9 PkS domains using the pDNA prime/protein boost protocol and stimulated in vitro with the corresponding recombinant protein antigen (5 µg/ml). Results represent mean ± SD IL-2 values (pg/ml) of 4–6 mice tested individually. Data are representative of one of three experiments.