Figure 5. Antigen-specific IFN-γ production in naïve and vaccinated mice, as tested by ELISPOT.

Number of IFN-γ producing cells in C57BL/6 mice vaccinated against the 9 PkS domains using the pDNA prime/protein boost protocol and stimulated in vitro with the corresponding recombinant protein antigen (5 µg/ml). Results represent mean ± SD of spot forming cells/10⁶ cells of 4–6 mice tested individually as detected in a 48 hr ELISPOT. Data are representative of one of two experiments. White columns: cells without Ag stimulation; black columns: cells stimulated for 48 hr with corresponding antigen.