Table 1. Primers used for the cloning of nine Pks domains.

Gene	Abbreviation	Primers for cloning in pV1J.ns-tPA	Enzyme used
Acyl carrier protein type 1	ACP-1	5′-aatAGATCTtgcacacccccgaaa-3′ 5′-attAGATCTtcatgtggggtgatcg-3′	Bgl II
Acyl carrier protein type 2	ACP-2	5′-aatAGATCTtggatcaagccgcatc-3′ 5′-gccAGATCTtcagggtgtg-3′	Bgl II
Acyl carrier protein type 3	ACP-3	5′-aatAGATCTtgcacacccccgaaa-3′ 5′-attAGATCTtcaggtgtgcaggtgttg-3′	Bgl II
Type 1 acyltransferase with acetate specificity	ATac-1	5′-tacTGATCAtgcgtctgtaccagcatctc-3′ 5′-gtaTGATCATcaatagtcaggtgaggcgagtt-3′	Bcl I
Type 2 acyltransferase with acetate specificity	ATac-2	5′-tatAGATCTtgtttgtgccctgggtgatttc-3′ 5′-tgaAGATCTtcagtggtccacccagtaac-3′	Bgl II
Acyltransferase with propionate specificity	ATp	5′-atcTGATCAtggcagtgggtgtactgg-3′ 5′-gatTGATCAtcacattgtggtggtgtcgt-3′	Bcl I
Enoylreductase	ER	5′-tacGGATCCtgcttgacaccaccggcaa-3′ 5′-gatGGATCCtcacatgtcacgtaaggccg-3′	BamH I
Type A ketoreductase domain	KR A	5′-aatAGATCTtgagggggaccgtgt-3′ 5′-attAGATCTtcaggcgagcgaatc-3′	Bgl II
Load module ketosynthase domain	KS	5′-ggccggtgAGATCTtgaattt-3′ 5′-attAGATCTtcagccacccatggaa-3′	Bgl II

Primers used for the cloning of nine Pks domains in plasmid pV1J.ns-tPA. Restriction enzymes used are indicated. Sense primers are listed first and antisense primers second, within primer pairs. All restriction sites are shown in bold.