Table 1. PCR and sequencing primers used in this study.

Primer	Nucleotide position (nt)	Primer sequence (5′–3′)	Orientation	Reference
EV71-VP1-S	2372–2392	GCAGCCCAAAAGAACTTCAC	Forward	[5]
EV71-VP1-A	3434–3454	AAGTCGCGAGAGCTGTCTTC	Reverse	[5]
CVA16-VP1-S	2335–2354	ATTGGTGCTCCCACTACAGC	Forward	[7]
CVA16-VP1-A	3426–3445	GCTGTCCTCCCACACAAGAT	Reverse	[7]
E486	2297–2322	TGGTAICARACIAAITWYGTIGTNCC	Forward	[22]
E488	3038–3063	GTIGGRTAICCITCITARAACCAYTG	Reverse	[22]
E490	2226–2248	TGIGTIYTITGYRTICCITGGAT	Forward	[22]
E492	2934–2953	GGRTTIGTIGWYTGCCA	Reverse	[22]
CVA10-2407Y	2407–2426	ACTGATGAGGTGACGCAACA	Forward	This study
CVA10-3400Z	3381–3400	CCAGGTGCCTATTGACCACT	Reverse	This study
CVB3-2289S	2289–2308	ACCGCAGGGGGTTTTATTAC	Forward	This study
CVB3-3748Q	3729–3748	ACTCCCTGTTCCATTGCATC	Reverse	This study