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. 2014 Jan 2;9(1):e84426. doi: 10.1371/journal.pone.0084426

Figure 4. Spot assays of Rosetta+pET 32a-ScCAT1 (a) and Rosetta+pET 32a (mock) (b) on LB plates with CuCl2, CdCl2 and NaCl (A–D).

Figure 4

And liquid culture assay in LB liquid medium with 750 µM CuCl2, 750 µM CdCl2 and 250 mM NaCl (E–H). IPTG (isopropyl β-D-thiogalactoside) was added to the cultures of Rosetta+pET 32a-ScCAT1 and Rosetta+pET 32a to induce the expression of recombinant protein. The cultures were adjusted to OD600 = 0.6. Ten microliters from 10−3 (left side of red line on plate) to 10−4 (right side of red line on plate) dilutions were spotted onto LB basal (A) plates or with CuCl2 (250, 500 and 750 µM) (B), CdCl2 (250, 500 and 750 µM) (C), NaCl (250, 500 and 750 mM) (D). For studying the growth analysis of ScCAT1, Rosetta+pET 32a-ScCAT1 and Rosetta+pET 32a were grown in LB liquid medium with LB basal medium (E) or with 750 µM CuCl2 (F), 750 µM CdCl2 (G), and 250 mM NaCl (H). All data points are means±SE (n = 3). CuCl2: copper chloride; CdCl2: cadmium chloride; NaCl: sodium chloride.