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. 2014 Jan 2;9(1):e84625. doi: 10.1371/journal.pone.0084625

Figure 6. Pyruvate Reduces the Detection of ROS Generated by Human Neutrophils.

Figure 6

(A) Human peripheral neutrophils were incubated with B. burgdorferi 5A4NP1 strain, ratio of 10∶1 B. burgdorferi:neutrophil, and ROS generation over 1 h was determined using the luminol detection assay and are expressed as Relative Light Units (RLU). (B) Human peripheral neutrophils were incubated with 5A4NP1, ratio of 10∶1 B. burgdorferi:neutrophil, and ROS generation over 1 h was determined using the luminol detection assay. Data shown are the integrated RLU for the duration of 3 separate experiments. Zymosan (SOZ) is a positive control for neutrophil activation and N-acetylcysteine (NAC) is a positive control for reduction of ROS. ROS produced by neutrophils with B. burgdorferi was set to 100% and the significance was compared to neutrophils+B. burgdorferi+antioxidant (pyruvate or NAC) using One-way ANOVA. Although percentages are shown, data were transformed to arcsine values using the formula SIN−1(√(%/100)) prior to One way ANOVA testing (* p<0.05). ROS produced by neutrophils with SOZ was set to 100% and significance was determined with One way ANOVA (* p<0.05) compared to the same condition with added antioxidant.