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. 2014 Jan 2;9(1):e84460. doi: 10.1371/journal.pone.0084460

Figure 7. Bmi1 regulates NPC proliferation and expression of p16 and Cdk1 mRNAs.

Figure 7

(A) Expression levels of necdin and Bmi1 in lentivirus-infected primary NPCs. Neocortical NPCs were prepared from wild-type (WT) and necdin-null (KO) mice at E14.5 and infected with lentiviruses for GFP expression (Control), Bmi1 overexpression (Bmi1 OE) and Bmi1 shRNA expression (Bmi1 KD). Infected NPCs were cultured for 48 hrs and harvested for Western blot analysis of Bmi1, necdin, and β-tubulin (β-Tub). (B) Proliferation assay. The S-phase cell population was analyzed 48 hrs after viral infection by BrdU incorporation assay. (C, D) Expression levels of p16 mRNA (C) and Cdk1 mRNA (D) were determined by qRT-PCR. Values in (B−D) represent the mean ± SEM, n = 3. *p<0.05, **p<0.01.