Table 1. List of differentially regulated proteins from a comparison of proton beam irradiated and unirradiated BLM cells.
| Spot noa | Accession nrb | Protein | MW [kDa] calculated | pI | MW [kDa]in gel | Scores | #Peptides | SCc [%] | Ratiod ir/ctrl | fold change | p-value |
| 1 | VIME_HUMAN | Vimentin | 53.6 | 4.9 | 52.6 | 2736.8 | 41 | 60.3 | 0.49 | −2.0 | 0.018 |
| 2 | VIME_HUMAN | Vimentin | 53.6 | 4.9 | 48.7 | 1866.5 | 29 | 51.9 | 0.46 | −2.2 | 0.031 |
| 3 | VIME_HUMAN | Vimentin | 53.6 | 4.9 | 49.7 | 2230.9 | 36 | 65.5 | 0.29 | −3.4 | 0.001 |
| 5 | VIME_HUMAN | Vimentin | 53.6 | 4.9 | 51.1 | 781.7 | 15 | 36.9 | 0.64 | −1.6 | 0.015 |
| 7 | TPIS_HUMAN | Triosephosphate isomerase | 30.8 | 5.6 | 31.4 | 889.2 | 10 | 52.1 | 0.46 | −2.2 | 0.017 |
| 8 | ANXA7_HUMAN | Annexin A7 | 52.7 | 5.4 | 55.0 | 637.2 | 8 | 15.0 | 0.40 | −2.5 | 0.032 |
| 46 | G3P_HUMAN | Glyceraldehyde-3-phosphate dehydrogenase | 36.0 | 9.3 | 35.0 | 932.7 | 47 | 24.0 | 0.42 | −2.4 | 0.003 |
| 30 | TERA_HUMAN | Transitional endoplasmic reticulum ATPase | 89.3 | 5.0 | 104.3 | 3308.2 | 46 | 59.3 | 1.89 | 1.9 | 0.015 |
| 31 | LMNB2_HUMAN | Lamin-B2 | 67.6 | 5.2 | 108.8 | 2304.7 | 35 | 48.3 | 1.38 | 1.4 | 0.005 |
| 32 | ACTN4_HUMAN | Alpha-actinin-4 | 104.8 | 5.2 | 106.7 | 4204.7 | 61 | 69.9 | 1.88 | 1.9 | 0.043 |
| 34 | CAPR1_HUMAN | Caprin-1 | 78.3 | 5.0 | 117.1 | 192.5 | 3 | 4.8 | 1.78 | 1.8 | 0.038 |
| 36 | MVP_HUMAN | Major vault protein | 99.3 | 5.2 | 112.6 | 1937.7 | 29 | 43.9 | 1.95 | 1.9 | 0.012 |
| 37 | FUBP2_HUMAN | Far upstream element-binding protein 2 | 73.1 | 7.0 | 94.6 | 1464.6 | 26 | 32.1 | 1.72 | 1.7 | 0.042 |
| 38 | ACTN1_HUMAN | Alpha-actinin-1 | 103.0 | 5.1 | 75.5 | 2786.0 | 43 | 59.2 | 1.85 | 1.8 | 0.049 |
| LMNA_HUMAN | Prelamin-A/C | 74.1 | 6.6 | 2069.0 | 32 | 42.9 | |||||
| 40 | PDC6I_HUMAN | Programmed cell death 6-interacting protein | 96.0 | 6.1 | 104.1 | 1739.9 | 25 | 37.9 | 1.55 | 1.6 | 0.029 |
| 41 | MCM7_HUMAN | DNA replication licensing factor MCM7 | 81.3 | 6.1 | 94.1 | 1574.3 | 25 | 37.0 | 1.66 | 1.7 | 0.047 |
| 42 | LMNA_HUMAN | Prelamin-A/C | 74.1 | 6.6 | 87.1 | 2285.0 | 30 | 47.0 | 1.59 | 1.6 | 0.028 |
| 43 | MOES_HUMAN | Moesin | 67.8 | 6.0 | 84.5 | 1807.4 | 29 | 42.6 | 2.36 | 2.4 | 0.013 |
| 44 | LMNA_HUMAN | Prelamin-A/C | 74.1 | 6.6 | 86.6 | 1881.7 | 23 | 37.3 | 2.45 | 2.5 | 0.027 |
| 45 | LMNA_HUMAN | Prelamin-A/C | 74.1 | 6.6 | 87.0 | 2508.5 | 33 | 50.8 | 1.46 | 1.5 | 0.033 |
| 47 | HSP71_HUMAN | Heat shock 70 kDa protein 1 | 70.0 | 5.4 | 64.5 | 1611.1 | 61 | 35.0 | 1.81 | 1.8 | 0.082 |
| G3BP1_HUMAN | Ras GTPase-activating protein-bindingprotein 1 | 52.1 | 5.3 | 969.4 | 55 | 24.0 | |||||
| 48 | STRAP_HUMAN | Serine-threonine kinase receptor-associated protein | 38.4 | 4.8 | 42.0 | 866.1 | 58 | 15.0 | 4.16 | 4.2 | 0.006 |
Spots were compared by 2D stained with Colloidal Coomassie and proteins were identified using LC-MS/MS.
a The spot location is shown in Figure 2 ad 3.
b Protein accession number from the UniProtKB/Swiss-Prot nonredundant protein database.
c obtained sequence coverage.
d Ratio calculated in relation to unirradiated control group.