Skip to main content
. 2014 Jan 2;10(1):e1003844. doi: 10.1371/journal.ppat.1003844

Figure 1. Colicin-dependent competition of S. Tm and E. coli in the gut in inflammation-induced “blooms” in gnotobiotic LCM mice.

Figure 1

Streptomycin-treated LCM-mice were co-infected with 1∶1 mixtures of S. TmΔoriT and EcMG1655, S. TmoriT Δcib and EcMG1655 or S. TmΔoriT avir and EcMG1655. (A) S. Tm (black) and E. coli (white) colonization densities were determined at day 4 p.i. in the cecum content (cfu/g). (B) Competitive indices (CI; ratio of S. Tm/E. coli) were determined for individual mice shown in (A). Bars show the median. (C) Histopathological analysis of cecal tissue of the infected mice shown in (A). Cecal tissue sections of the mice were stained with hematoxylin/eosin (H&E) and the degree of submucosal edema, neutrophil infiltration, epithelial damage and loss of goblet cells was scored (Materials and Methods). 1–3: no pathological changes; 4–7: moderate inflammation; above 8: severe inflammation. shown mean and StD. (D) Representative H&E–stained cecal sections of mice shown in (A–C). Magnification 100-fold. Enlarged sections (squares) are shown in the lower panels. Dotted line: detection limit (1 cfu/g).