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. 2014 Jan 2;10(1):e1003854. doi: 10.1371/journal.ppat.1003854

Figure 4. Over-expression of a dominant-negative version of PfSET2 in cultured parasites.

Figure 4

(A) Schematic diagrams showing the domain structures of the endogenous PfSET2 protein (top) and the truncated, dominant-negative version that contains only the N-terminal 359 amino acids (bottom), including the CTD-binding domain (SRIR). (B) Histograms showing RNA expression levels of the endogenous PfSET2 gene (PfSET2) and the dominant-negative construct (PfSRIR) under 2 and 10 µg/ml blasticidin. Two separate subclones of NF54 were transfected, A3 (left) and C3 (right). (C and D) Pie charts representing var gene expression patterns for A3 (left) and C3 (right) when over-expressing either firefly luciferase (C) or the PfSET2 dominant-negative construct (D). The different wedges of the pie indicate the proportion of the total var mRNA pool represented by transcripts for each individual gene (as described in [28]).