Figure 4. M2 induction of IRF4 by M2 is partially dependent on NFAT pathway.
(A) DS10 and C30p cells were either left uninduced or induced with or without CsA for 48 hours in the culture media. Whole cell lysates were harvested at 48 h post induction and 5×105 cell equivalents were analyzed for levels of IRF4 protein by immunoblotting. PMA and Ionomycin treatment for 6 hours served as a positive control for IRF4 induction. P3X63Ag8 cell lysates served as a stimuli independent positive control. (B) Duplicate gel run using whole cell lysates from (A) to verify expression of M2 using antibody for AU1 tag. (C) Supernatants from cells in Figure 4A were analyzed for IL-10 levels by ELISA.