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. 2014 Jan 2;9(1):e84248. doi: 10.1371/journal.pone.0084248

Figure 3. Annexin–V FITC/PI assay.

Figure 3

MDA-MB-231 cells were treated with the complexes AuD6 and AuD8 (20 µM) for 16 and 24 h. Then, cells were labeled with Annexin–V FITC and PI and analyzed by flow cytometry in order to evaluate the percentage of apoptotic cells. Apoptotic cells at early stage occur in the lower right quadrant while apoptotic cells at late stage set in the up-right part. The percentage in the lower left quadrant is due to viable cells whereas the upper left part to non-apoptotic cell death.