Figure 5. Western blot in vivo analysis.

Female nude mice bearing MDA-MB-231 tumors were treated with either vehicle (control) or the compounds AuD6 and AuD8 at 1 mg kg⁻¹ d⁻¹. Tumors were collected and the corresponding tissues prepared for Western blot analysis after either 13-day treatment (A) or 27-day treatment (B) [I and II denote distinct experiments]. Tissues were also prepared for the assays of caspase-3 activity (C) and of proteasomal CT-like activity (D) after 27 days of treatment.