Table 3.
Bacterial density at 45 DAI under gnotobiotic conditions
|
Treatments
1
|
Endophytic population (log cfu/g)
2
|
||
|---|---|---|---|
| Root | Stem | Leaves | |
| Wild type |
7.83 ± 0.10a |
7.13 ± 0.05a |
0 |
|
nifHΔ |
7.62 ± 0.16a |
7.02 ± 0.19a |
0 |
|
nifDΔ |
7.67 ± 0.20a |
6.91 ± 0.32a |
0 |
|
nifKΔ |
7.65 ± 0.14a |
6.98 ± 0.32a |
0 |
| Mock | 0 | 0 | 0 |
1Wt and nif mutants were labelled with pMC1-EPS-RtGFP and inoculated to root zones of 12 day-old seedlings derived from surface-sterilized. Plants were maintained in sterile conditions in sand-nutrient mix in Phytatrays.
2Number of CFU (per g dry biomass) recovered from surface-sterilized root tissues of Jatropha curcas cv. MD-44. Bacteria were recovered on 2xYT medium supplemented with chloramphenical (100 μg/ml).
aThe treatments are not significantly different from each other at 5% threshold.