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. 2013 Oct 1;3:23. doi: 10.1186/2044-5040-3-23

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Copyright © 2013 Apponi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nuclear poly(A) binding protein 1 (PABPN1) levels are low in all skeletal muscles. Lysates prepared from different (A) mouse tissues (50 μg of total protein per lane), (B) mouse muscles (150 μg of total protein per lane) or (C) human tissues (20 μg of total protein per lane) were immunoblotted with anti-PABPN1 antibody. Histone H3 and heat shock protein 90 (HSP90) were used as loading controls for mouse samples. Amido black staining was used as the loading control for human samples. Immunoblots are representative of at least three independent sets of tissues.