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Proton Translocation Activity of Lysine Swap Mutants.
| Mutant | λmax | Lysine Linkage Helix | Relative Initial Rate |
|---|---|---|---|
| WT | 548 | G | 1.0 |
| A53K/K216A | 520 | B | 0.11 |
| A53K/K216V | 510 | B | ND |
| A53K/K216I | 510 | B | ND |
| T89K/K216T | 600 | C | ND |
Note.—Rates are given relative to WT H. turkmenica bR. ND indicates no detectable activity in our assays.
