FIGURE 5.

Role of the TA dinucleotide at the insertion site. A, TCC assembly was done as indicated in Fig. 1A using a TA target or a GC target (which contains no TA dinucleotide) and analyzed by EMSA. The analysis was repeated five times, and the percentage of TCC for each target was obtained by dividing the amount of TCC by the amount of TCC + free target. The normalized results were plotted on a graph. B, integration assays were performed as described for Fig. 4, using cold PC-ITR and a labeled TA target or a labeled GC target. Integration products were analyzed onto denaturing gel.