FIGURE 6.

Increased RNAPII CTD phosphorylation by Tip110 and Tat. 293T cells were transfected with an increasing amount of pTip110.HA (A), pTat.Myc (B), or pTat.Myc (0.5 μg) plus an increasing amount of pTip110.HA (C). ΔNT mutant (G; marked by an asterisk) was also included. pcDNA3 was added to equalize the total amounts of plasmid DNA transfected. Whole cell extracts were prepared 72 h post-transfection and subjected to Western blot analysis with α-Tip110, α-Myc, α-β-actin, α-CTDa, α-CTDo2, or α-CTDo5 antibody. The relative levels of CTDa, CTDo2, and CTDo5 in A, B, C, and G were quantitated by densitometry, and quantitative data from multiple independent experiments are presented in D, E, F, and H.