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Effects of hypoxia (5% O2 and 0% O2) on SGLT2 mRNA and protein expression in LLC-PK1 cells. LLC-PK1 monolayers were incubated in 5% O2 (A and C) and 0% O2 (B and D) for 1, 3, 6, and 12 h. SGLT2 mRNA and protein were evaluated by PCR and Western blotting, respectively (*, p < 0.05 versus t = 0 h). Values are means ± S.D. (error bars) for six independent experiments, each in duplicate.
