FIGURE 8.
PfRh4 interactions with sCR1, LHR A, and m7–9,18–20r. CR1 constructs were injected at multiple concentrations over surface plasmon resonance chip immobilized PfRh4 (850 RU). A parallel flow path bearing 630 RU of immobilized mouse IgG was used as a reference. Dissociation constants were calculated from Req values derived from reference-subtracted curves and fitted to a Langmuir 1:1 interaction model. A, sCR1 was injected at 10, 6.7, 3.3, 2.2, 0.7, 0.2, and 0.08 μm. The KD (indicated by a dotted line) was calculated to be 8 ± 1 μm. B, supernatants from transfected 293T containing LHR A were injected at 5, 1.7, 0.6, 0.2, and 0.1 μm. KD = 2 ± 1 μm. C, supernatants from transfected 293T cells containing the m7–9,18–20r protein were injected in duplicate at 250, 128, 64, 32, and 17 nm. KD = 61 ± 9 nm, ∼30-fold greater than that observed for PfRh4:LHR A.