FIGURE 4.
Comparison of FRET efficiencies of NOsGC mutants. A, FRET efficiency (%) under basal conditions for NOsGC wild-type enzyme and NOsGC mutants are compared. B, change in FRET efficiency (%) after the addition of DEA/NO (100 μm) for NOsGC wild-type enzyme and NOsGC mutants. Values were calculated as the difference of basal FRET efficiency to NO-stimulated FRET efficiency. An asterisk marks p value < 0.05 compared with wild-type enzyme. The results are expressed as means ± S.E. of at least three independent experiments.