FIGURE 2.

The release of GlcA and MeGlcA from different xylans by BoAgu115A. In panel A, wild type (WT) Arabidopsis or the mutant gux1gux2 (which does not decorate xylans with [Me]GlcA) were treated with BoAgu115A. In panel B, the xylans extracted from the four plants were subjected to PACE in the absence (−) or presence (+) of BoAgu115A. *, non-enzyme dependent product. In panel C, wild type Arabidopsis was treated with a GH30 glucuronoxylanase alone (WT + BoGH30), prior to treatment with BoAgu115A (WT + BoGH30 + BoAgu115A), or subsequent to treatment with BoAgu115A (WT + BoAgu115A + BoGH30). The glucurono-xylooligosaccharides displayed in panel C are as follows: UX₃, XXUX; UX₄, XXXUX; UX₅, XXXXUX; UX₆, XXXXXUX.