TABLE 2.
Kinetics of wild type BoAgu115A against glucuronoxylans and glucurono-xylooligosaccharides
Data are averages and S.D. from three independent experiments.
| Substrate | kcat | Km | kcat/Km |
|---|---|---|---|
| min−1 | mm | min−1 mm−1 | |
| XUXX | 3233 (±187) | 4.5 (±0.54) | 718 |
| UXX | 1106 (±71) | 19.5 (±1.90) | 57 |
| Beech wood xylan | 696 (±38) | 0.4 (±0.06) | 1740 |
| Birch wood xylan | 665 (±24) | 0.3 (±0.04) | 2217 |
| Beech wood xylan /GH30a | 837 (±77) | 0.9 (±0.15) | 930 |
| Birch wood xylan/GH30a | 862 (±64) | 1.1 (±0.15) | 784 |
a The polysaccharide was pre-digested to completion with a GH30 glucuronoxylan-specific xylanase.