The N-terminal 118 amino acids are not involved in intramolecular complex formation of murine TACC3.
A, primary structure of TACC3-ΔN lacking the N-terminal part of 118 aa. B, aSEC (Superpose 6, 10/300) elution profiles of GST-TACC3-ΔN before (solid line) and after (dashed line) thrombin cleavage. Vo, void volume. mAu, milliabsorbance units. C, peak elution fractions were analyzed by SDS-PAGE followed by CBB staining (upper panel) and immunoblotting using anti-TACC3 C18 (middle panel) or anti-GST antibodies (lower panel). GST was used as a control.