FIGURE 6.

The C terminus of chTOG following the six TOG domains binds to the CC1 subdomain of TACC3. The interaction between the C terminus of chTOG (divided into the subfragments chTOG-A and chTOG-B; supplemental Fig. S5) with full-length TACC3 or its CC1 subdomain was analyzed by pulldown assays and subsequently detected by CBB staining and immunoblotting using antibodies against the C-terminal end of TACC3 (C18). chTOG-B, but not the TOG6 domain containing fragment chTOG-A (supplemental Fig. S5), displays a selective interaction with TACC3.