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. 2013 Nov 22;289(1):74–88. doi: 10.1074/jbc.M113.532333

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FIGURE 7. — Binding of chTOG-Cterm to TACC3 disrupts the intradomain interaction of TACC3. A, the interaction between chTOG-Cterm and thrombin-cleaved TACC3 was analyzed by pulldown assays and immunoblotting using the indicated antibodies. Intramolecular interaction of TACC3-N7R with CC is thereby abrogated upon chTOG-Cterm binding, as chTOG-Cterm pulls down CC but not TACC3-N7R (last lane). Thr., thrombin. B and C, aSEC-based analysis of the TACC3 - chTOG-Cterm complex was performed employing uncleaved TACC3 (B) or thrombin-cleaved TACC3 either alone or prebound to chTOG-Cterm (C). Peak fractions were analyzed by SDS-PAGE and CBB staining. The dashed lines within the CBB-stained SDS-PAGE gels highlight the peak shift of the chTOG-Cterm-CC complex (but not of TACC3-N7R) after thrombin cleavage. mAu, milliabsorbance units.