Fig 1.

The expression of TRPM8 in osteosarcoma tissues and cell lines. A: TRPM8 is over-expressed in osteosarcoma tissues when compared with osteochondroma tissues used as normal control (magnification x400), arrows indicate the positive staining cells. Negative controls for immunostaining were performed in the absence of anti-TRPM8 antibody. The correlation of TRPM8 expression in osteosarcoma and osteochondroma specimens was assessed by chi-square test. B: The expression of TRPM8 in osteosarcoma cell lines was detected by western blot (a) and its abundance was expressed as normalized values over BMSCs (b). C: The knockdown efficiency of siRNA targeted on TRPM8 in MG-63 and U2OS cells. (a) Ca²⁺ imaging indicated that the response to menthol was clearly diminished in siTRPM8 cells and the knockdown of TRPM8 leads to impaired regulation of intracellular Ca²⁺ concentration. The fluorescence intensity in y-axis represents intracellular Ca²⁺ concentration. (b) The time course and dose dependent manner of TRPM8 siRNA in MG-63 and U2OS cells. (c) The western blot data in (b) was quantified and the results were expressed in histograms.