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. 2013 Dec 22;10(1):90–102. doi: 10.7150/ijbs.7738

Fig 2.

Fig 2

Knockdown of TRPM8 suppressed growth of MG-63 and U2OS cells. A: The effect of TRPM8 knockdown on MG-63 (a) and U2OS (b) cell proliferation was measured by CCK8 assay. Growth of siTRPM8 cells was significantly suppressed at day 2 compared with Parental and siCON cells. One-Way ANOVA was used for the data analysis. **P<0.01. OD in y-axis means A450nm and represents cell growth; bars, SD. B: Parental, siCON and siTRPM8 cells of MG-63 (a) and U2OS (b) were immunostained with anti-Ki67 antibody (green) and Hoechst33258 (blue), scale bars = 50 μm.