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. 2013 Dec 22;10(1):90–102. doi: 10.7150/ijbs.7738

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 7 — The changes of p-p44/p42 and pJNK. A: After transfected with siCON and siTRPM8, MG-63 (a) and U2OS (b) cells were treated with 500ng/ml EPI for the indicated time and then western blot was performed to investigate the p-p44/p42 and p44/p42. (c) The results of the western blot were quantified and expressed in histograms. B: (a) After 48h EPI treatment, p-p44/p42 was analyzed in each sample by western blot. (b) The quantitative data of the western blot and statistic analysis was performed by One-Way ANOVA, ^**P<0.01,^*P<0.05. C: Cells were prepared as indicated in material and methods, and p-JNK and JNK was analyzed by western blot. Knockdown of TRPM8 had no influence on the phosphorylation of JNK (a); however, after EPI treatment it enhanced the phosphorylation of JNK (b). (c) The results of western blot in (b) were quantified and One-Way ANOVA was applied for the statistic analysis, ^*P<0.05. D: (a) p-MEK, MEK and MKP-1 was investigated by western blot in Parental, siCON and siTRPM8 cells. (b) The results of western blot were quantified and One-Way ANOVA was applied for the statistic analysis, ^*P<0.05.