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Representative MS/MS spectra of COS-1 p53. Peptides prepared from p53 isolated from COS-1 cells were subjected to reversed-phase nano-LC-MS and MS/MS on a UPLC-Orbitrap Velos platform as described under “Experimental Procedures.” Shown are representative examples of tandem mass spectra displaying their PTM-bearing peptide fragment ion assignments, labeled as described in the legend to Fig. 4.
